[Histonet] Kappa/Lambda ISH on Ventana

[Angela Bitting]

Ventana can no longer give out protocols. Theses probes are ASR products and the FDA doesn't allow them to tell you the protocol. They can offer guidance if you have a protocol that doesn't work well, but they can't outright tell you a protocol. Another institution that runs Ventana probes is your best bet. 
 
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> "Sebree Linda A" <LSebree <@t> uwhealth.org> 3/31/2011 7:09 PM >>>
You should be able to get protocols directly from Ventana.  I tried out
their K and L ISH probes and they gave us the protocols.  They also
supplied us with the protocol we use for EBER ISH. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Wilkes
Sent: Thursday, March 31, 2011 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Kappa/Lambda ISH on Ventana

Hi Histonet!

I have been tasked with validating Kappa and Lambda on our Ventana
Benchmark
XT machines.  Does anyone who uses this platform have suggestions as to
which Ventana protease works best (1, 2 or 3)?  Additionally, any other
information concerning the optimization (suggested incubation times, RNA
regions etc.) would be greatly appreciated.  Thanks in advance.

Steven
srwilkes <@t> gmail.com 
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