[Histonet] histomorphometry

[Yak-Nam Wang]

Hi Louise,

If your lab is like other labs and are a bit strapped for money but need
basic point counting, you may also be able do this using ImageJ. ImageJ is a
free piece of software developed at and provided by NIH. It has grid and
point overlays and counting tools. The learning curve isn't very steep, and
it's free! 

http://rsbweb.nih.gov/ij/

There is also a pc version.

Good luck
Yak-Nam Wang


On 9/28/10 1:47 PM, "Wagner, Richard [BUL/LAK]" <Richard.Wagner <@t> BUEHLER.COM>
wrote:

> Louise:
> 
> Versatile image analysis software like OmniMet can solve this problem.
> Powerful digital image acquisition, object and field measurement
> options, and counting tools are standard features of the software.
> http://www.buehler.com/productinfo/biomedical/ia.htm
>  
> Regards,
> Rick Wagner
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jack
> Ratliff
> Sent: Tuesday, September 28, 2010 8:36 AM
> To: louise renton
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] histomorphometry
> 
> At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva
> demonstrated this very technique in his workshop. My best advice would
> be to contact him directly (avillanueva11 <@t> cox.net) and see what he can
> do for you. Another option would be to contact an image analysis vendor
> like BIOQUANT (nathanael <@t> bioquant.com). Hope this information helps!
> 
> Regards,
> 
> Jack
> 
> On Sep 28, 2010, at 3:25 AM, louise renton <louise.renton <@t> gmail.com>
> wrote:
> 
>> Hi all,
>> 
>> I desperately need some advice from an experienced
> histomorphometrist....I
>> am trying to translate the old cumbersome visual measurement of using
> an
>> eypiece grid and doing point counting to an easier computer system -
> but I
>> am not sure how to do it - any help out there?
>> -- 
>> Louise Renton
>> Bone Research Unit
>> University of the Witwatersrand
>> Johannesburg
>> South Africa
>> +27 11 717 2298 (tel & fax)
>> 073 5574456 (emergencies only)
>> "There are nights when the wolves are silent and only the moon howls".
>> George Carlin
>> No trees were killed in the sending of this message.
>> However, many electrons were terribly inconvenienced.
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> 
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