[Histonet] rat kidney problems

[Geoff McAuliffe]

Greetings Abi:

For cortical tubules (proximal convoluted tubules, PCT) to have a nice, 
open lumen one needs to fix by perfusion.This has been known for many years.
Failing that, use a fix that works very rapidly in needed, alcoholic 
formalin for example, and slice the kidney into thin slices.
What happens with less than optimal fixation is the brush border of the 
PCT collapses into the lumen, filling it.
Regions that do not have a well developed brush border do not have this 
problem, as you see.
Why you are seeing this "only now", I do not know.

Geoff

abijag wrote:
> Hello
>
> I need your advice regarding paraffin sections of rat kidney. Kidneys are collected, adequately fixed with 10 % NBF and undergone routine tissue processing in Sakura VIP 6 tissue processor.Sections are of 5 micron thickness. Strangely, in the H&E stained sections, our pathologists are complaining that typical morphology of tubules are not preserved ie. typical tubular patterns are missing (tubules with no lumen) in the cortical region (at the edges and slightly inwards). But the inner medulla and other regions are perfectly fine.This issue cropped up now only.Any body will give their expert advice to solve this issue.
>
>
>
> Thanks
>
>
>
> Abi  
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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