[Histonet] Her2 question

[Fawn Bomar]

Hi everyone,

I was wondering how many of you all use the Cell Marque Her2 predilute?  We have been using it at our facility for 2 years now and it has been great until we received a new lot.  This new lot does not seem to be staining as well and I was wondering if anyone else was having the same problem and if they managed to get it to stain better.  I called Cell Marque and they told me that they optimized the antibody again so it is different from what we have been using.  I am adjusting a few things on our protocol to see if it works and I was wondering if anyone had any tips.

We use the BenchMark LT and our protocol is as follows:

Deparaffinization, Standard CC1, Antibody incubation 32 minutes (the maximum that the machine allows), A/B Block, Hematoxylin-6 minutes, and bluing-4 minutes.

Thank you
Fawn Bomar HT(ASCP)
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Thank you