[Histonet] RE: Cutting, Processing, etc

[Nails, Felton]

 what is happening to our field??????????????

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Senn, Amy R
Sent: Thursday, September 16, 2010 10:38 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cutting, Processing, etc

Hello Histoland!

 

I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here...

 

After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade?

How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same?

 

How often is "freezy" spray used in your lab, and where and when do you use it?

 

How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use?

 

Thank you so much for your input!!

 

Amy Senn, HT

Holy Spirit Hospital, Camp Hill PA

 

 

 

 

 

 

 



 

 

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