[Histonet] Cutting, Processing, etc

[Senn, Amy R]

Hello Histoland!

 

I have some questions about procedures in different histo labs and I'd
like to have some 'backup' when people look at me like I'm crazy here...

 

After embedding, you face (trim) your blocks, right? Do you take
sections right from that same blade, or move/change your blade?

How many of you 'soak' your blocks in water/softblock before cutting
them? Do you put them on a cold plate/use ice and water, etc? Does this
depend on the type of tissue, or do you treat them all the same?

 

How often is "freezy" spray used in your lab, and where and when do you
use it?

 

How often do you rotate/change your reagents in your processors? Do you
calculate this by how many blocks/days/weeks of use?

 

Thank you so much for your input!!

 

Amy Senn, HT

Holy Spirit Hospital, Camp Hill PA

 

 

 

 

 

 

 



 

 

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