[Histonet] Losing frozen sections

[Fischer, Dolores]

Hello again,

Besides my C3 and C5b-9 question I was wondering if anyone has ideas as to why I might be losing my frozen sections during IHC.   I'm placing 5u sections on charged slides, air drying overnight, fixing in chilled acetone, air drying again, and proceeding with the protocol, which recently has been an ABC method.   Slides I don't use get stored in a -70 freezer.   I do these by hand.  Could I just be rinsing too vigorously?  Sometimes I look at these during the procedure and they look fine, but inevitably many of the sections look horrible- torn and missing tissue, by the time they are coverslipped.   Hints and suggestions anyone?

Thank you in advance,


Dolores
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