[Histonet] Barcoding

Tue Sep 14 13:03:46 CDT 2010

Our software (NiceLabel / NiceWatch) has the ability to take in data from any system. Data can be text, XML, HL7, JOB files, open formatted files, etc. We can connect via TCP/IP, Web Services, COM Port or a file drop. The data is them parsed out and place onto a label template formatted in NiceLabel Pro. A GUI design tool. We support all barcode symbologies (1D and 2D). We can then output to any printer that has a Windows print driver. Thermal, Laser, Line Matrix, Ink Jet or devices like slide and cassette printers. Please contact me if you would like additional information, a demonstration or trial of our software.

Thank you,

Maggie Allen
Healthcare Business Development Manager
Niceware International, LLC
Tel  (810) 629-3930
Cell (215) 200-0268
Email: maggie.allen <@t> nicewareintl.com
www.nicewareintl.com
http://healthcare.nicewareintl.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, September 09, 2010 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 82, Issue 11

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Today's Topics:

   1. H&E+ Alcian Blue (Deborah Faichney)
   2. RE: bar coding specimens, slides, blocks (Tom McNemar)
   3. FW: [Histonet] Odd question (Morken, Tim)
   4. RE: bar coding specimens, slides, blocks (Feher, Stephen)
   5. RE: Pathology billing for consultation (Feher, Stephen)
   6. Re: FW: [Histonet] Odd question (Kim.Donadio <@t> bhcpns.org)
   7. giving back tissue (Tench, Bill)
   8. Re: bar coding specimens, slides, blocks (Langenberg, Stacey)
   9. HT position in Colorado  (Hale, Meredith)
  10. tissue refixation ? (Benoit Loup)
  11. Re: H&E+ Alcian Blue (Bryan Llewellyn)

----------------------------------------------------------------------

Message: 1
Date: Thu, 9 Sep 2010 09:44:39 +0100
From: Deborah Faichney <d.a.faichney <@t> stir.ac.uk>
Subject: [Histonet] H&E+ Alcian Blue
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <8ED3F2CA5B78E142B8193376C57330F8EAE96449C5 <@t> EXCH2007.ad.stir.ac.uk>
Content-Type: text/plain; charset="us-ascii"

Hello all,

I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5.    I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin.  I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes.   (Thus giving 3 slides stained with:  AB, AB+H and AB+H+E)  The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain.  I am expecting the alcian blue to remain turquoise: should it?

For information, I am trying to stain salmon intestine at 5 microns for using the following:

Alcian Blue 8GX (certified), pH has been checked
Haematoxylin Z (Cellpath Uk)
1% aq Eosin (Cellpath uk) and lab prepared solution from dye.

Thanks from a frustrated technician!

Debbie Faichney
Histopathology
Institute of Aquaculture
University of Stirling
Scotland
UK

--
The Sunday Times Scottish University of the Year 2009/2010
The University of Stirling is a charity registered in Scotland,
 number SC 011159.

------------------------------

Message: 2
Date: Thu, 9 Sep 2010 06:04:06 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: bar coding specimens, slides, blocks
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,
        "HISTONET <@t> PATHOLOGY.SWMED.EDU"  <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
        <E9A90E28259D2F4E84308C5E8EA8F7B4A51D7C7B <@t> lmhs-exchange.lmhealth.org>
Content-Type: text/plain; charset="us-ascii"

Also interested in this...

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, September 08, 2010 4:08 PM
To: HISTONET <@t> PATHOLOGY.SWMED.EDU
Subject: [Histonet] bar coding specimens, slides, blocks

I am looking for a vendor that has the capability to barcode specimens, blocks and slides.   Also if it can interface with Meditech client server 6.0 it would be a plus.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

------------------------------

Message: 3
Date: Thu, 9 Sep 2010 08:48:05 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: FW: [Histonet] Odd question
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1AAF670737F193429070841C6B2ADD4C0255010115 <@t> EXMBMCB15.ucsfmedicalcenter.org>

Content-Type: text/plain; charset=us-ascii

This link will take you to the article about legalisms of tissue and blocks:

http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12

Tim

>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 9/8/2010 6:49 PM >>>

There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org).

It also addresses wet tissue, other things taken out of people.

The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials."

So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Wednesday, September 08, 2010 3:18 PM
To: 'Kim.Donadio <@t> bhcpns.org'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Odd question

Consult your medical legal department on this issue because this is not new situation.
It is often times based on the person religion but the request should be made know at the time of surgery.
So pathology can be made aware and avoid putting the specimen in formalin.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim.Donadio <@t> bhcpns.org
Sent: Wednesday, September 08, 2010 4:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Odd question

Hi Histonetters,
                                 Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology?

I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste.

Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin?

Help and thanks in advance :-)

Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

-----------------------------------------
All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring.
This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message.  For questions, contact the BHC Privacy Officer at (850) 434-4472.  Rev.10/07.
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------------------------------

Message: 4
Date: Thu, 9 Sep 2010 12:03:55 -0400
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] bar coding specimens, slides, blocks
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,
        <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
        <73A7ED895EE0C24D9267ED814911DF191773F461 <@t> exchange.cmc-nh.org>
Content-Type: text/plain;       charset="us-ascii"

We have had a great deal of success using Leica's IPC and IPS cassette
and slide labelers.  We use Soft Path but I had heard that the Leica LIS
experts have experience interfacing with a number of LIS systems.  We
set these up basically as just another printer that is interfaced with
our LIS system.  The software within the Leica labelers produces the
cassettes and slides with any configuration of accession number and/or
bar code that you would like.  We chose to use 2d barcode to save space
on the cassette and slide.  We also print our ThinPrep slides this way.
Leica and Hologic got together to make sure we were able to use the
correct coding to use these slides on our ThinPrep Imager.

Steve

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn,
Hazel V
Sent: Wednesday, September 08, 2010 4:08 PM
To: HISTONET <@t> PATHOLOGY.SWMED.EDU
Subject: [Histonet] bar coding specimens, slides, blocks

I am looking for a vendor that has the capability to barcode specimens,
blocks and slides.   Also if it can interface with Meditech client
server 6.0 it would be a plus.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription Arkansas Children's
Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

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are hereby notified that any dissemination, distribution or copying of
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Thank you.
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------------------------------

Message: 5
Date: Thu, 9 Sep 2010 12:06:21 -0400
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] Pathology billing for consultation
To: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>,
        <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
        <73A7ED895EE0C24D9267ED814911DF191773F462 <@t> exchange.cmc-nh.org>
Content-Type: text/plain;       charset="us-ascii"

There are a couple of ways to handle this and most of them have already
been mentioned.  We have a contract Pathology group in our hospital so
if the patient insurance does not pay, the consultant bills the
Pathology Group.  Unless your pathologists are completely sold on their
expert consultants, outside labs such as Genzyme will do the third party
billing if you use their consultants.  I know that they accept insurance
assignment and have relationships with most large insurers so the
patient rarely gets stuck with a bill.

Steve

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Demarinis, Carolyn
Sent: Wednesday, September 08, 2010 9:22 AM
To: HISTONET <@t> PATHOLOGY.SWMED.EDU
Subject: [Histonet] Pathology billing for consultation

I would like to know how other pathology labs are billing for
consultations that are sent out by pathologist for second opinion.
Our process is to notify the physician's office that a case is being
sent to an expert and, if required, the physician's office is
responsible for obtaining precertification if the patient's insurance
require it.
Unfortunately, this has caused us a number of problems.  If the
consultant is not "in-network", the insurance does not cover this
expense, and the patient is responsible for the bill.
Is it a better option for the hospital to receive all bills from
consultants, and in turn, the hospital will bill the patient?  If so,
are there problems associated with this?
Or are other laboratories having the consultants bill the patient's
insurance directly, and if so, are they experiencing similar problems?
Thanks.
Carolyn DeMarinis, Pathology Supervisor
Saratoga Hospital Laboratory

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------------------------------

Message: 6
Date: Thu, 9 Sep 2010 11:12:55 -0500
From: Kim.Donadio <@t> bhcpns.org
Subject: Re: FW: [Histonet] Odd question
To: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,
        histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
        <OF9458E274.25CA7447-ON86257799.0059060A-86257799.005912B9 <@t> bhcpns.org>
Content-Type: text/plain;       charset="US-ASCII"

Thank you all for responding. All of you were very helpful.

Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

"Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
09/09/2010 10:48 AM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
FW: [Histonet] Odd question

This link will take you to the article about legalisms of tissue and
blocks:

http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12

Tim

>>> "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org> 9/8/2010 6:49 PM >>>

There was a good article about this topic in Lab Medicine (ASCP) "Who Owns
Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73
(available in pdf format on line at www.ascp.org).

It also addresses wet tissue, other things taken out of people.

The key sentence in that article is: "In practice, there are no specific
laws, case law, or prior legal rulings that explicitly address ownership
of diagnostic materials."

So, it is left up to individual institutions to develop their own
guidelines. Obviously, then, there will be many variations of practice.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nails,
Felton
Sent: Wednesday, September 08, 2010 3:18 PM
To: 'Kim.Donadio <@t> bhcpns.org'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Odd question

Consult your medical legal department on this issue because this is not
new situation.
It is often times based on the person religion but the request should be
made know at the time of surgery.
So pathology can be made aware and avoid putting the specimen in formalin.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kim.Donadio <@t> bhcpns.org
Sent: Wednesday, September 08, 2010 4:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Odd question

Hi Histonetters,
                                 Can anyone give me any idea of any laws
that guide giving a patient there organ after we have taken from it what
we need to do the Histology?

I know we have to keep it for a minimum of two weeks after sign out ( our
policy is 6 weeks after sign out ). But then we dispose of it as medical
waste.

Are any of you aware of any guidelines on giving a patient there entire
organ which would be submerged in formalin?

Help and thanks in advance :-)

Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

-----------------------------------------
All electronic data transmissions originating from or sent to Baptist
Health Care Corporation (BHC) are subject to monitoring.
This message along with any attached data, are the confidential and
proprietary communications of BHC and are intended to be received only by
the individual or individuals to whom the message has been addressed. If
the reader of this message is not the intended recipient, please take
notice that any use, copying, printing, forwarding or distribution of this
message, in any form, is strictly prohibited and may violate State or
Federal Law. If you have received this transmission in error, please
delete or destroy all copies of this message.  For questions, contact the
BHC Privacy Officer at (850) 434-4472.  Rev.10/07.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------------------------------------
CONFIDENTIALITY NOTICE:
 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.
============================================================

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 7
Date: Thu, 9 Sep 2010 09:14:12 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] giving back tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A55D6 <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii

In all of this discussion, it is important to understand that there are
significant variations in state laws that relate to this issue, so if
this problem arises, have your hospital/lab attorney check into state
laws very carefully.  In California, the laboratory may not "own" the
tissue (again, depending on releases never read but signed by the
patient on admission) but it clearly is the responsible "custodian."
This applies to sending out slides and blocks for research and for
clinical testing.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

[None] made the following annotations
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Message: 8
Date: Thu, 9 Sep 2010 10:16:11 -0600
From: "Langenberg, Stacey" <STACEY.LANGENBERG <@t> UCDENVER.EDU>
Subject: Re: [Histonet] bar coding specimens, slides, blocks
To: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>,
        "histonet-bounces <@t> lists.utsouthwestern.edu"
        <histonet-bounces <@t> lists.utsouthwestern.edu>, "Horn, Hazel V"
        <HornHV <@t> archildrens.org>, "HISTONET <@t> PATHOLOGY.SWMED.EDU"
        <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
        <99316908.1375278.1284048976798.JavaMail.rim <@t> bda2340.bisx.prod.on.blackberry>

Content-Type: text/plain; charset="iso-8859-1"

We too use both the IPS and IPC. These are on such an open system we created our own LIS and now have barcoding from grossing to slide signout.

Stacey
Sent via BlackBerry from T-Mobile

-----Original Message-----
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Sender: "histonet-bounces <@t> lists.utsouthwestern.edu"
        <histonet-bounces <@t> lists.utsouthwestern.edu>
Date: Thu, 9 Sep 2010 10:03:55
To: Horn, Hazel V<HornHV <@t> archildrens.org>; HISTONET <@t> PATHOLOGY.SWMED.EDU<HISTONET <@t> PATHOLOGY.SWMED.EDU>
Subject: RE: [Histonet] bar coding specimens, slides, blocks

We have had a great deal of success using Leica's IPC and IPS cassette
and slide labelers.  We use Soft Path but I had heard that the Leica LIS
experts have experience interfacing with a number of LIS systems.  We
set these up basically as just another printer that is interfaced with
our LIS system.  The software within the Leica labelers produces the
cassettes and slides with any configuration of accession number and/or
bar code that you would like.  We chose to use 2d barcode to save space
on the cassette and slide.  We also print our ThinPrep slides this way.
Leica and Hologic got together to make sure we were able to use the
correct coding to use these slides on our ThinPrep Imager.

Steve

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn,
Hazel V
Sent: Wednesday, September 08, 2010 4:08 PM
To: HISTONET <@t> PATHOLOGY.SWMED.EDU
Subject: [Histonet] bar coding specimens, slides, blocks

I am looking for a vendor that has the capability to barcode specimens,
blocks and slides.   Also if it can interface with Meditech client
server 6.0 it would be a plus.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription Arkansas Children's
Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org

************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
************************************************************************
******************************************************
The information contained in this message may be privileged and
confidential and protected from disclosure. If the reader of this
message is not the intended recipient, or an employee or agent
responsible for delivering this message to the intended recipient, you
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Message: 9
Date: Thu, 9 Sep 2010 11:21:24 -0500
From: "Hale, Meredith" <mhale <@t> carisls.com>
Subject: [Histonet] HT position in Colorado
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: "Roupp, Kevin" <kroup <@t> carisls.com>
Message-ID:
        <6F33D8418806044682A391273399860F051F3FA5 <@t> s-irv-ex301.PathologyPartners.intranet>

Content-Type: text/plain;       charset="us-ascii"

Colorado GI Pathology (CGIP) is seeking an experienced histotech to
complement its existing staff. CGIP is a specialized lab located in
Centennial, CO that provides services to the three largest
gastroenterology practices in Denver. Dr. Russell Nash is the chief
pathologist and medical director. Tracy Wrinkle is the Operations
Manager. The position can be filled by an experienced person who can
work from 32 to 40 hours per week. The schedule is basically days,
although coverage for other times during vacations will be needed. The
pay range starts at $26.00 per hour with benefits. This is a small,
congenial work place with an emphasis on team work and cooperation. If
you are interested in learning more about the opportunity, please
contact Tracy Wrinkle at twrinkle <@t> cogipath.com or call her at (303)
770-4848 for a confidential interview and tour of the facility.

Meredith Hale HT (ASCP) CM

Operations Liaison Director and Education Coordinator

Caris Life Sciences

6655 North MacArthur Blvd, Irving Texas 75039

direct: 214-596-2219

cell: 469-648-8253

fax: 972-929-9966

mhale <@t> carisls.com

------------------------------

Message: 10
Date: Thu, 09 Sep 2010 18:23:04 +0200
From: Benoit Loup <benoit.loup <@t> jouy.inra.fr>
Subject: [Histonet] tissue refixation ?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4C8909E8.7040800 <@t> jouy.inra.fr>
Content-Type: text/plain; charset=windows-1252; format=flowed

  Hi to all,

I have some troubles with H/E staining of tissues fixed in Bouin's.
In fact it worked very well during my first assays and now my tissue
sections are torn and looks like bad.
I think that the fixation time was not sufficient (4h for 3mmx2mmx2mm
pieces). Thus is it possible to dewax, rehydrate and refix tissue in
bouin's before sectioning ?
I also have some pieces already sectioned and mounted on slide but not
yet dewaxed and stained. Is it also possible to refix these sections or
are they definitively lost ?

Thanks to all for your comments and help.

Benoit

--
Benoit Loup, PhD
UMR Biologie du D?veloppement et Reproduction
Diff?renciation des Gonades et Perturbations
INRA ? Domaine de Vilvert
B?timent Jacques Poly
78350 Jouy en Josas
France

Tel: 33 1 34 65 25 38
Fax: 33 1 34 65 22 41
E-mail: benoit.loup <@t> jouy.inra.fr

------------------------------

Message: 11
Date: Thu, 9 Sep 2010 09:43:03 -0700
From: "Bryan Llewellyn" <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] H&E+ Alcian Blue
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A9F1B4E2D2F9459AB21796C0951102CD <@t> BryanPC>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
        reply-type=original

You are probably using a regressive hematoxylin.  Most of those stain acid
mucins purple-blue.  To overcome it use a strictly progressive hematoxylin
such as Mayer (hx 1 g., alum 50 g.) for 5 minutes.  The order should be
alcian blue, wash, H&E.

Bryan Llewellyn

----- Original Message -----
From: "Deborah Faichney" <d.a.faichney <@t> stir.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 09, 2010 1:44 AM
Subject: [Histonet] H&E+ Alcian Blue

Hello all,

I have a request to carry out a combined staining with H&E + Alcian Blue
pH2.5.    I have tried in vain to get this to work but regardless of the
order of staining the end result is dark blue/purple mucin.  I have carried
out a parallel experiment whereby the staining has been checked
microscopically then stopped after each of the dyes.   (Thus giving 3 slides
stained with:  AB, AB+H and AB+H+E)  The AB and AB+H are really nicely
stained but as soon as the eosin is added (using 2 different stains and a
variety of times) the mucin staining looks similar to the nuclear stain.  I
am expecting the alcian blue to remain turquoise: should it?

For information, I am trying to stain salmon intestine at 5 microns for
using the following:

Alcian Blue 8GX (certified), pH has been checked
Haematoxylin Z (Cellpath Uk)
1% aq Eosin (Cellpath uk) and lab prepared solution from dye.

Thanks from a frustrated technician!

Debbie Faichney
Histopathology
Institute of Aquaculture
University of Stirling
Scotland
UK

--
The Sunday Times Scottish University of the Year 2009/2010
The University of Stirling is a charity registered in Scotland,
 number SC 011159.

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End of Histonet Digest, Vol 82, Issue 11
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