[Histonet] poor collagen staining on skins

[Clare Thornton]

Hello all,

Our skin sections occasionally contain collagen that looks "burnt", to quote the pathologist.  We use a Symphony stainer and Ventana has been here frequently trying to figure out the cause of the problem to no avail.  Does anyone have any ideas?  Could it be due to inadequate deparaffinization, inadequate baking, section thickness, slide issues, any of the above?  We are just looking for some new ideas and fresh viewpoints!

Thanks,
Clare

Clare J. Thornton, HTL(ASCP),QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com