[Histonet] macroscopic demonstration of lung mets in rodent lung

[Madary, Joseph]

Hi netters, I am thinking an acetic based fixative would be used to
inlfate the mouse lung to make the metastatic portions stand out next to
normal tissue..  An investigator wants to do a lung inflation with a
fixative that will allow him to view the lung mets and count areas as he
breadloafs the lung(even though it is small).  Bouins or Davidsons
maybe?  He asks if India ink perfusion would work too.  Anyone try that?
Any feedback is appreciated.

 

Nick Madary, HT/HTL(ASCP)QIHC

Medimmune Histology Mgr, 

OMW, Area 4, Lab 2438

301.398.4745(vm)

301.398.6360(lab)

301.398.9745(fax)

 




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