[Histonet] H&E+ Alcian Blue

[Kim.Donadio <@t> bhcpns.org]

It wont remain turquoise as long as you use the eosin. At least this is my 
experience. I have known some Pathologist to read the mucin that way 
(purple). 




Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Deborah Faichney <d.a.faichney <@t> stir.ac.uk> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
09/09/2010 03:44 AM

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Subject
[Histonet] H&E+ Alcian Blue






Hello all,

I have a request to carry out a combined staining with H&E + Alcian Blue 
pH2.5.    I have tried in vain to get this to work but regardless of the 
order of staining the end result is dark blue/purple mucin.  I have 
carried out a parallel experiment whereby the staining has been checked 
microscopically then stopped after each of the dyes.   (Thus giving 3 
slides stained with:  AB, AB+H and AB+H+E)  The AB and AB+H are really 
nicely stained but as soon as the eosin is added (using 2 different stains 
and a variety of times) the mucin staining looks similar to the nuclear 
stain.  I am expecting the alcian blue to remain turquoise: should it?

For information, I am trying to stain salmon intestine at 5 microns for 
using the following:

Alcian Blue 8GX (certified), pH has been checked
Haematoxylin Z (Cellpath Uk)
1% aq Eosin (Cellpath uk) and lab prepared solution from dye.

Thanks from a frustrated technician!

Debbie Faichney
Histopathology
Institute of Aquaculture
University of Stirling
Scotland
UK








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