[Histonet] RE: Saponin Technique

[IRENA SREBOTNIK KIRBIS]

for removing  erythrocytes from hemorrhagic cytology samples we use filtration of bloody suspension through membrane filter with pore size 20 mikrons, you can find an article in Cytopathology. 2007 Jun;18(3):175-9. Epub 2007 Mar 27.
it is worth trying!
regards
Irena
 
> From: TMcNemar <@t> lmhealth.org
> To: Valerie.Hannen <@t> parrishmed.com; histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 25 Oct 2010 11:52:31 -0400
> CC: 
> Subject: [Histonet] RE: Saponin Technique
> 
> I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution. We get the balanced salt solution from our pharmacy.
> 
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
> Sent: Monday, October 25, 2010 11:40 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] FW: Saponin Technique
> 
> 
> 
> ________________________________
> 
> From: Hannen, Valerie
> Sent: Monday, October 25, 2010 11:39 AM
> To: 'histonet <@t> list.utsouthwestern.edu'
> Subject: Saponin Technique
> 
> 
> Hi folks..
> I am hoping someone out there in Histo-land can help. One of our
> Pathologists is asking us to check into doing the Saponin Technique to
> lyse the red cells in some of our cytology specimens. She gave a copy of
> one of the pages from one of her manuals, and it shows that in order for
> us to do this we would need to buy alot of reagents that we currently do
> not have. Being that we would probably not be doing this technique very
> often, and not sure how cost effective it will be for us to buy the
> reagents, we are wondering if anyone knows of a commercial product that
> we can buy that would be a cheaper way out.
> 
> Thanks in advance!!
> Valerie Hannen, Histotechnologist
> Parrish Medical Center
> Titusville,Florida
> 
> 
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