[Histonet] RE: Saponin Technique

Tom McNemar TMcNemar <@t> lmhealth.org
Mon Oct 25 10:52:31 CDT 2010

I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution.   We get the balanced salt solution from our pharmacy.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, October 25, 2010 11:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: Saponin Technique


From: Hannen, Valerie
Sent: Monday, October 25, 2010 11:39 AM
To: 'histonet <@t> list.utsouthwestern.edu'
Subject: Saponin Technique

Hi folks..
I am hoping someone out there in Histo-land can help. One of our
Pathologists is asking us to check into doing the Saponin Technique to
lyse the red cells in some of our cytology specimens. She gave a copy of
one of the pages from one of her manuals, and it shows that in order for
us to do this we would need to buy alot of reagents that we currently do
not have. Being that we would probably not be doing this technique very
often, and not sure how cost effective it will be for us to buy the
reagents, we are wondering if  anyone knows of a commercial product that
we can buy that would be a cheaper way out.

Thanks in advance!!
Valerie Hannen, Histotechnologist
Parrish Medical Center

"This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you
have received this communication in error, please immediately
delete this message. Thank you"
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

More information about the Histonet mailing list