[Histonet] Nitroblue Tetrazolium Chloride

louise renton louise.renton <@t> gmail.com
Fri Oct 29 02:04:28 CDT 2010

I used to do a "stain" on post mortem heart slices to look for recent
infarctions. the entire tissue turned a sort of bluey colour which remained
through formain fixation and processing. the tissue however was incubated in
 solution of NBT in a sodium cyanide containing buffer - not sure if that
would still be allowed these days - but if you want - i could send you the
protocol we used

best regards

On Thu, Oct 28, 2010 at 8:51 PM, Laurie Colbert <
laurie.colbert <@t> huntingtonhospital.com> wrote:

> I previously posted a question regarding Nitroblue Tetrazolium Chloride,
> but I didn't really receive the info that I needed - so I thought I
> would ask again.
> I need to purchase this item for a research doc.  He wants to immerse
> tissue in this solution for 24 hours and then process as usual.  It is
> my understanding that this is some kind of dye.  I see that I can order
> it from Sigma in tablet form.  I've seen it from other companies in a
> powder form.
> Has anyone ever used this reagent in the capacity that I describe?  Is
> it available as a ready-to-use solution/liquid?  Is there a certain
> strength/percentage that I should use?
> Thanks,
> Laurie Colbert
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Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
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