[Histonet] Re: xylene substitute

[Jennifer MacDonald]

xylene substitutes also require more time than xylene





"Johnson, Teri" <TJJ <@t> stowers.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/27/2010 10:13 AM

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[Histonet] Re: xylene substitute






Hi all,

I just wanted to let folks know that if you plan to switch to a xylene 
substitute, realize that the substitutes are more intolerant of water than 
xylene is. Your last alcohol will have to be as anhydrous as you can get 
it or you will have water carryover that may cause some problems. If you 
live in a humid climate, this might end up being problematic for you.

Having said that, we prefer using ProPar or Clear-Rite 3 (aliphatic 
hydrocarbon reagents) over the limonenes.

Carry on!

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO


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