[Histonet] Re: xylene substitute

Johnson, Teri TJJ <@t> stowers.org
Wed Oct 27 12:10:35 CDT 2010

Hi all,

I just wanted to let folks know that if you plan to switch to a xylene substitute, realize that the substitutes are more intolerant of water than xylene is. Your last alcohol will have to be as anhydrous as you can get it or you will have water carryover that may cause some problems. If you live in a humid climate, this might end up being problematic for you.

Having said that, we prefer using ProPar or Clear-Rite 3 (aliphatic hydrocarbon reagents) over the limonenes.

Carry on!

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO

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