[Histonet] Coverslip removal question

[Rene J Buesa]

No, once you have removed the coverslip, just wash the section thoroughly in xylene to remove any thick remnant of the old permount, and reapply permount and cover.
René J.

--- On Fri, 10/22/10, Lewis, Patrick <patrick.lewis <@t> seattlechildrens.org> wrote:


From: Lewis, Patrick <patrick.lewis <@t> seattlechildrens.org>
Subject: [Histonet] Coverslip removal question
To: Histonet <@t> lists.utsouthwestern.edu
Date: Friday, October 22, 2010, 3:55 PM


Hi Histonetters



When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.

And then drying and re applying permount?



Or can I go straight from xylene to drying to re-coversliping with
permount?







Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

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