[Histonet] Coverslip removal question

Lewis, Patrick patrick.lewis <@t> seattlechildrens.org
Fri Oct 22 14:55:20 CDT 2010

Hi Histonetters


When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.

And then drying and re applying permount?


Or can I go straight from xylene to drying to re-coversliping with




Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

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