[Histonet] Coverslip removal question

[Lewis, Patrick]

Hi Histonetters

 

When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.

And then drying and re applying permount?

 

Or can I go straight from xylene to drying to re-coversliping with
permount?

 

 

 

Patrick Lewis

Research Associate II-Bench| Infections and Prematurity

Seattle Children's Research Institute

206-884-1115  OFFICE 

000-000-0000  PAGER

000-000-0000  CELL 

206-884-7311  FAX

patrick.lewis <@t> seattlechildrens.org

OFFICE  1900 9th Avenue Seattle, WA 98101

MAIL      M/S C9S-8, Seattle, WA 98101

WWW     seattlechildrens.org <http://seattlechildrens.org/> 

 



CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.