AW: [Histonet] kappa and lambda by CISH

[Gudrun Lang]

I've found, that kappa and lambda mRNA CISH is reliable for specimen
decalcified with 5-10% formic acid. Decal with harsher acids like
hydrochloric acid should be validated, because any acid will cause
degradation of nucleic acids. We use the CISH kit from Zytovision.
Journal of Histotechnol 33(1):9-13, 2010

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Kuhnla,
Melissa
Gesendet: Dienstag, 19. Oktober 2010 16:50
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] kappa and lambda by CISH

Hello All,
Anyone currently running Kappa and Lambda by CISH?  I am in the process
of validating the Ventana products.  Due to the ASR status of these
products the vendor is extremely 'tight lipped'.  I have the stains
worked up and have completed a 25 case validation. Does anyone know if
this test is not to be used on decaled specimens??
Thank you


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