[Histonet] negative controls

BSullivan <@t> shorememorial.org BSullivan <@t> shorememorial.org
Fri Oct 15 11:00:39 CDT 2010

We're talking NEGATIVE controls here folks and if you use internal tissue
for your positive and negative controls...........they should all be
processed and blocked in the same fashion as your actual patient material.
We have scrutinized the negative control issue on the CAP checklist and
have decided that if tissue warrants it, we cut an extra patient slide and
it is run as the negative for that case. All is processed the same and
stained identically with exception of the primary antibody. If there is not
adequate material  we use the Positive control and treat it negatively.
This is reflected in our policy and procedure.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital

             <sgoebel <@t> xbiotech                                             
             Sent by:                                                   To 
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                                       , Sebree Linda A                    
             10/15/2010 11:47          <LSebree <@t> uwhealth.org>              
             AM                                                    Subject 
                                       RE: [Histonet] negative controls    

So for every HP you do, you process a control cassette with the patient
tissue cassette?  That seems like alot?  How do you get that many
control tissues on a daily basis?  What do you do with the remaining
tissue in the control block?  If you throw them away everyday, I would
be interested in some of them.  How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time?  We have always just had a bunch of
blocks that you cut a control from?  I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue.  If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times.  Why couldn't you
just have one for all 3 cases?  Then the next day have a fresh ONE for
that day, date them, and file them.  So if you needed to see the HP
control for October 15th, you could go pull the control for that day...

Sarah Goebel, B.A., HT (ASCP)

XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744


-------- Original Message --------
Subject: RE: [Histonet] negative controls
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A <LSebree <@t> uwhealth.org>, sgoebel <@t> xbiotech.com
Cc: Histo Net list server <HistoNet <@t> lists.utsouthwestern.edu>

Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.

--- On Fri, 10/15/10, sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com> wrote:

From: sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com>
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Cc: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
Date: Friday, October 15, 2010, 11:17 AM

   Why do you need a negative control for each block if you are runn=
   the  same  antibody  on each patient block?  Is it just for case by c
  ase  reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all
   slides you d= id on that day, on that run, could be referenced back
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744


   (512)386-= 5107

   -------- Original Message --------
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]LSebree@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= oria <@t> gmail.com>, "Histo Net
   <[3]HistoNet <@t> lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   -----Original Message-----
   From: [4]histonet= -bounces <@t> lists.utsouthwestern.edu
   [[5]mailto:histon=  et-bounces <@t> lists.utsouthwestern.edu]  On Behalf
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   I have a hypothetical question to those who run IHC on Ventana
   Are you running your negatives with your patient/test cases or on a
   run? Also, if you are doing this and have to use a different
   how do you work the QA/QC portion of this for CAP requirements.
   Histonet mailing list
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