[Histonet] negative controls

Mark Tarango marktarango <@t> gmail.com
Fri Oct 15 10:56:57 CDT 2010 

Hi Sarah,

It's better to have the control on the same slide.  There are slides that
work and slides that don't.  You know which ones are good and which ones are
bad because you have that control on each slide.  It's not always a complete
run that fails.  Yes, you CAN have a single batch control (it's not against
the rules), but best practice is to have a control on each slide.

You don't process a control for each case, you use a similarly processed
piece of positive tissue placed on each slide (except the negative which
should just have the patient tissue).


Mark
On Fri, Oct 15, 2010 at 8:47 AM, <sgoebel <@t> xbiotech.com> wrote:

> So for every HP you do, you process a control cassette with the patient
> tissue cassette?  That seems like alot?  How do you get that many
> control tissues on a daily basis?  What do you do with the remaining
> tissue in the control block?  If you throw them away everyday, I would
> be interested in some of them.  How do you know what IHC stains the
> pathologist is going to order to know what control tissue to fix and
> process at the exact same time?  We have always just had a bunch of
> blocks that you cut a control from?  I understand that there is
> variability with processing, age, etc. not trying to be dense just still
> don't understand... Most places I have ever worked have control blocks
> that they cut a fresh control from everyday, then stain with the patient
> tissue.  If there are 3 HP cases, from what I am understanding, you guys
> are saying you need 3 controls for slides that are on the same machine,
> with the same reagents, same antibody, and same times.  Why couldn't you
> just have one for all 3 cases?  Then the next day have a fresh ONE for
> that day, date them, and file them.  So if you needed to see the HP
> control for October 15th, you could go pull the control for that day...
>
> Sarah Goebel, B.A., HT (ASCP)
> Histotechnician
>
>
> XBiotech USA Inc.
>
> 8201 East Riverside Dr. Bldg 4 Suite 100
>
> Austin, Texas  78744
>
> (512)386-5107
>
>
>
>
> -------- Original Message --------
> Subject: RE: [Histonet] negative controls
>  From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Date: Fri, October 15, 2010 8:33 am
> To: Sebree Linda A <LSebree <@t> uwhealth.org>, sgoebel <@t> xbiotech.com
> Cc: Histo Net list server <HistoNet <@t> lists.utsouthwestern.edu>
>
> Because each tissue block has its own characteristics regarding fixation
> and processing some of which can influence the reactivity. If you have a
> bank of negative controls, how can you be sure that any of those blocks
> have received exactly the same treatment and reacted in the same way to
> the test block?
> The same goes for any bank of positives, so that is why you should have
> a positive control section in the same slide as the test section.
> René J.
>
> --- On Fri, 10/15/10, sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com> wrote:
>
>
> From: sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com>
> Subject: RE: [Histonet] negative controls
> To: "Sebree Linda A" <LSebree <@t> uwhealth.org>
> Cc: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
> Date: Friday, October 15, 2010, 11:17 AM
>
>
>   Why do you need a negative control for each block if you are runn=
> ing
>   the  same  antibody  on each patient block?  Is it just for case by c
>  ase  reference  so  the negative is filed with the patient slide?  Why
>   co=  uldn't  you  have  a control slide bank that was dated so all
> the
>   slides you d= id on that day, on that run, could be referenced back
> to
>   that control? = ; Just curious?
>
>   Sarah Goebel, B.A., HT (ASCP)
>
>   Histotechnician<= br>
>
>   XBiotech USA Inc.
>
>   8201 East Riverside Dr. Bld= g 4 Suite 100
>
>   Austin, Texas  78744
>
>   =
>
>   (512)386-= 5107
>
>   -------- Original Message --------
>   Subject: RE: [Histonet] negative controls
>   From: "Sebree Linda A" <[1]LSebree@= uwhealth.org>
>   Date: Fri, October 15, 2010 8:08 am
>   To:  "Victoria  Baker"  <[2]bakevict= oria <@t> gmail.com>, "Histo Net
> list
>   server"
>   <[3]HistoNet <@t> lists.uts= outhwestern.edu>
>   We run negative controls on every block of a case within the same
> run.
>   On autopsy cases, we only run 1 negative per tissue type, within the
>   same run...this is the only exception to the rule of 1 negative per
>   block.
>   Linda A. Sebree
>   University of Wisconsin Hospital & Clinics
>   IHC/ISH Laboratory
>   DB1-223 VAH
>   600 Highland Ave.
>   Madison, WI 53792
>   (608)265-6596
>   -----Original Message-----
>   From: [4]histonet= -bounces <@t> lists.utsouthwestern.edu
>   [[5]mailto:histon=  et-bounces <@t> lists.utsouthwestern.edu]  On Behalf
> Of
>   Victoria
>   Baker
>   Sent: Friday, October 15, 2010 9:26 AM
>   To: Histo Net list server
>   Subject: [Histonet] negative controls
>   Hi
>   I have a hypothetical question to those who run IHC on Ventana
>   instruments.
>   Are you running your negatives with your patient/test cases or on a
>   separate
>   run? Also, if you are doing this and have to use a different
> detection
>   kit
>   how do you work the QA/QC portion of this for CAP requirements.
>   Thanks
>   Vikki
>   _______________________________________________
>   Histonet mailing list
>   [6]Histonet <@t> lists.utsouth= western.edu
>   [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
>   _______________________________________________
>   Histonet mailing list
>   [8]Histonet <@t> lists.utsouth= western.edu
>   [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> References
>
>   1. 3D"mailto:LSebree <@t> uwhealth.org"
>   2. 3D"mailto:bakevictoria <@t> gmail.com"
>   3. 3D"mailto:HistoNet <@t> lists.utsouthwestern.edu"
>   4. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
>   5. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
>   6. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
>   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
>   8. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
>   9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

More information about the Histonet mailing list