[Histonet] negative controls

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Oct 15 10:33:37 CDT 2010

Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block?
The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section.
René J. 

--- On Fri, 10/15/10, sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com> wrote:

From: sgoebel <@t> xbiotech.com <sgoebel <@t> xbiotech.com>
Subject: RE: [Histonet] negative controls
To: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Cc: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
Date: Friday, October 15, 2010, 11:17 AM

   Why do you need a negative control for each block if you are runn= ing
   the  same  antibody  on each patient block?  Is it just for case by c   ase  reference  so  the negative is filed with the patient slide?  Why
   co=  uldn't  you  have  a control slide bank that was dated so all the
   slides you d= id on that day, on that run, could be referenced back to
   that control? = ; Just curious?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician<= br>

   XBiotech USA Inc.

   8201 East Riverside Dr. Bld= g 4 Suite 100

   Austin, Texas  78744


   (512)386-= 5107

   -------- Original Message --------
   Subject: RE: [Histonet] negative controls
   From: "Sebree Linda A" <[1]LSebree@= uwhealth.org>
   Date: Fri, October 15, 2010 8:08 am
   To:  "Victoria  Baker"  <[2]bakevict= oria <@t> gmail.com>, "Histo Net list
   <[3]HistoNet <@t> lists.uts= outhwestern.edu>
   We run negative controls on every block of a case within the same run.
   On autopsy cases, we only run 1 negative per tissue type, within the
   same run...this is the only exception to the rule of 1 negative per
   Linda A. Sebree
   University of Wisconsin Hospital & Clinics
   IHC/ISH Laboratory
   DB1-223 VAH
   600 Highland Ave.
   Madison, WI 53792
   -----Original Message-----
   From: [4]histonet= -bounces <@t> lists.utsouthwestern.edu
   [[5]mailto:histon=  et-bounces <@t> lists.utsouthwestern.edu]  On Behalf Of
   Sent: Friday, October 15, 2010 9:26 AM
   To: Histo Net list server
   Subject: [Histonet] negative controls
   I have a hypothetical question to those who run IHC on Ventana
   Are you running your negatives with your patient/test cases or on a
   run? Also, if you are doing this and have to use a different detection
   how do you work the QA/QC portion of this for CAP requirements.
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