[Histonet] Optimal processing for prostate needle biopsies

[Phyllis Thaxton]

Nilesh,
 This doesn't sound like a staining problem, it sounds like a processing 
problem. Try preprocessing for 5 minutes instead of 15 and see if that helps. OR 
what we had to do with GI biopsies, we stopped using any preprocessing with 
them. After fixation, they are placed in molecular fixative for 10 minutes 
before processing. 

  If that doesn't work, I would investigate further to where the biopsy was 
actually obtained. We had a problem with urologists obtaining the core, then 
swishing the needle gun around in a container of saline, then ADDING formalin to 
the saline after the biopsy was obtained. The biopsies were horrible.
  Hope this helps.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 




________________________________
From: "Gupta, Nilesh" <NGUPTA1 <@t> hfhs.org>
To: Phyllis Thaxton <dchihc <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, October 14, 2010 8:08:17 AM
Subject: RE: [Histonet] Optimal processing for prostate needle biopsies


Phyllis, 
We hold all our needle cores overnight and pre-process for 15 min. The problems 
we are having is shrunken nuclei and nucleoli are not distinct even in carcinoma 
cases. Cracking artifacts of cores (longitudinal), darker and paler staining 
along the length of the cores. We tried Harris but staining with Mayer's brings 
out nucleoli better.
 
 

________________________________
From: Phyllis Thaxton [dchihc <@t> yahoo.com]
Sent: Wednesday, October 13, 2010 10:37 AM
To: Gupta, Nilesh; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Optimal processing for prostate needle biopsies


How long are you pre-processing the prostate biopsies before processing on the 
XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, 
then wash for 20 minutes, pre-processing solution for 10 minutes then process. 
We use Harris Hematoxylin in our H&E and morphology is good.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 




________________________________
From: "Gupta, Nilesh" <NGUPTA1 <@t> hfhs.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, October 12, 2010 12:02:23 PM
Subject: [Histonet] Optimal processing for prostate needle biopsies

I have a few questions regrading processing of prostate needle biopsies.
1.  What is optimal fixation time that the needle cores should be fixed for 
before loading these on the processors.
2.  Our cores are processed on Tissue tek Xpress x120 but the morphology is not 
so good. I'd like to know if any other labs have tried this processor for 
prostate needle cores processing and would like to know their experience as far 
as morphologic quality on slides
3.  What is the best H&E stain to use for prostate needle biopsies. We are 
currently using Mayer's which is giving us better staining than Harris. Any 
recommendations on which H&E is best suited for prostate needle biopsies.

Thanks

N.Gupta
Henry Ford Hospital

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