[Histonet] Optimal processing for prostate needle biopsies

[Gupta, Nilesh]

Phyllis,
We hold all our needle cores overnight and pre-process for 15 min. The problems we are having is shrunken nuclei and nucleoli are not distinct even in carcinoma cases. Cracking artifacts of cores (longitudinal), darker and paler staining along the length of the cores. We tried Harris but staining with Mayer's brings out nucleoli better.


________________________________
From: Phyllis Thaxton [dchihc <@t> yahoo.com]
Sent: Wednesday, October 13, 2010 10:37 AM
To: Gupta, Nilesh; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Optimal processing for prostate needle biopsies

How long are you pre-processing the prostate biopsies before processing on the XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin in our H&E and morphology is good.

Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL


________________________________
From: "Gupta, Nilesh" <NGUPTA1 <@t> hfhs.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, October 12, 2010 12:02:23 PM
Subject: [Histonet] Optimal processing for prostate needle biopsies

I have a few questions regrading processing of prostate needle biopsies.
1.  What is optimal fixation time that the needle cores should be fixed for before loading these on the processors.
2.  Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides
3.  What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies.

Thanks

N.Gupta
Henry Ford Hospital

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