[Histonet] Advanced workshop in 3D live cell imaging in Sydney on 16-19 November.

[Anya Salih]

> You and your students are invited to attend the 3rd Advanced
> Bio-Imaging Workshop at the University of Western Sydney on 16 - 19
> November, 2010 and the Bio-Imaging Expo (free event on 16th November
> 2010) 
> 
> Tracking Molecules with Light
> 
> Training in confocal imaging and protein 3D tracking, aggregation,
> diffusion analyses. Experiments will involve mammalian cell lines,
> invertebrate (coral) cells, plant and algae, bacteria and other
> samples.
> 
> Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. 
> 
> Places limited to 35 and only 20 places left so register now.
> 
> 
> Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury
> Campus, University of Western Sydney
> Organiser: Dr Anya Salih
> Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ.
> California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker &
> Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss)
> 
> 
> Lectures and intensive hands-on training on confocal microscopes by
> top researchers in the field. Learn how to explore and analyse the 3D
> structural complexity of invertebrate, animal & plant cells, tissues
> and micro-organisms, visualize and analyse movement of organelles and
> molecules. Trial a range of novel GFP-type protein constructs. Discuss
> your experiments and trial new approaches. Workshop emphasis on
> advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B,
> FLIM, photoactivatable fluorescent proteins.
> 
> Invited speakers
> 
> *       Professor Enrico Gratton, Director Laboratory for Fluorescence
> Dynamics, University of California, Irvine
> *       Professor Takeharu Nagai, Laboratory for Nanosystems
> Physiology & Nikon Imaging Center, Hokkaido University Research
> Institute Electronic Science, Japan
> *       Dr. Michelle Digman, Director Optical Biology Core Facility,
> University of California, Irvine
> *       A/Professor Guy Cox, Australian Centre for Microscopy &
> Microanalysis, University of Sydney
> *       Professor Leann Tilley, Department of Biochemistry, D/Director
> Centre of Excellence in coherent X-ray Science, La Trobe University
> *       Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging
> Facility, Garvan Institute of Medical Research, University of Sydney
> *       Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute,
> University of Sydney
> *       Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin
> 
> Training sessions cover the following:
> 
> Multi-colour Fluorescent proteins
> Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby,
> pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B
> histone, actin, tubulin, Golgi, membrane); novel Photoactive
> Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from
> reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein
> localization & diffusion.
> 
> Confocal Spectral Imaging
> Acquisition of microspectral data (x, y, lambda) in 3D image stacks
> from samples with multiple fluorescent probes or from fluorescent
> coral tissues expressing a variety of GFP-type proteins (A. Salih
> fluorescent corals
> http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he
> alth)- Spectral unmixing, analysis, spectral FRET.
> 
> Analysis of molecular movement and diffusion 
> Track proteins and other molecules in live cells. Measure protein
> femtoliter concentrations. Monitor mobility and binding using
> fluorescence correlation spectroscopy (FCS), scanning FCS, raster
> image correlation spectroscopy (RICS), number & brightness (N&B) and
> photon counting histogram (PCH).
> 
> Fluorescence Lifetime Imaging Microscopy (FLIM)
> - a powerful tool to analyse spatial distribution of excited state
> lifetimes in samples: studied examples will include FRET-FLIM to study
> protein interactions (e.g., DNA-Protein) in cells, imaging of
> photoactivatable FPs, quenching of chlorophyll in plants, etc.
> Hands-on training in FLIM and Phasor FLIM.
> 
> 
> Workshop microscopes & companies
> 
> Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto
> optical beam   splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B
> Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging,
> photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid
> image acquisition, resonant scanner & 32 channel microspectral
> detection Olymus FluoView FV1000 - variable barrier filter (VBF),
> spectral detection, RICS and N&B
> Ultra   VIEW VoX spinning disc confocal microscope, high speed,
> multichannel,   2D and 3D, FRAP, FLIP, photoactivation experiments,
> PerkinElmer.
> Confocal FLIM system, Becker &  Hickl GmbH, Berlin A range of new
> GFP-type protein  constructs of many colours (cyan to far red) linked
> to a variety of cellular  proteins for in vivo protein localization
> and dynamic studies, Evrogen And many more other instruments.
> 
> 
> Registration
> 
> Full workshop registration (lectures + training) will be limited to 35
> participants.
> Registration will be on a first come first served basis.
> 
> Contact Anya Salih a.salih <@t> uws.edu.au to reserve your workshop place
> Students   $650 (GST inclusive)
> All other  $850 (GST inclusive)
> 
> 
> Attendance of the BioImaging Expo on 16th November does not require
> registration by please rsvp Pamela McMurtry
> [pamela.mcmurtry <@t> theconferenceteam.com.au]
> Accommodation details at registration website, from $260 per week per
> single room at UWS College.
> Bus shuttle will be available between Sydney airport and the workshop
> venue at UWS campus.
> 
> Kind regards,
Anya

Dr Anya Salih
Confocal Bio-Imaging Facility
University Western Sydney
Australia
> 61 2 45701452
> a.salih <@t> uws.edu.au