[Histonet] RE: New Cap Guidelines for Her2 and ER/PR

Mahoney,Janice A Janice.Mahoney <@t> alegent.org
Tue Oct 12 10:24:54 CDT 2010

We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time.  We do work on Saturdays so it is only the rare 3 day weekends that this comes into play.
Jan M

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joyce Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR

Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing?  I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time.  Does 70% affect antigenicity for either Her2 or ER/PR?  Any information or suggestions will be greatly appreciated.  Thanks :)

Ronda Souders
Hagerstown Medical Laboratory
fax 301-665-4941
ronda.souders <@t> wchsys.org<mailto:ronda.souders <@t> wchsys.org>

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