[Histonet] Bone IHC

Kuhnla, Melissa Melissa.Kuhnla <@t> chsli.org
Fri Oct 8 05:38:28 CDT 2010

Are you placing this bone section on a pre-cut control slides?  The
section will adhere better on its own slide.

Are you drying your slides at room temp prior to baking?  It always
helps to remove all trapped water from under the section prior to

Post fixing with formalin fumes is said to help.  Remove slides from
oven. Place in a rack in a staining cup w formalin just covering the
bottom of the cup (not covering the section). Cover and allow the fumes
to 'post fix' for 15 minutes.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa
J. Phelan
Sent: Thursday, October 07, 2010 3:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone IHC

Hi everyone,

I was wondering if anyone has any tricks on how to get bone sections to
stop lifting off the slide through the IHC process? I leave them in the
oven for quite a while to make sure they are baked on, however after
antigen retrieval  (pressure cooker for 20mins) most of the boney part
of the tissue comes off and the marrow and muscle stays put! The
sections are cut onto superfrost plus slides.

Any help would be much appreciated, thanks.

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