[Histonet] chicken EGFP aby

[Adam .]

I've had good luck using Abcam's chicken polyclonal:
http://www.abcam.com/GFP-antibody-ab13970.html

I use a directly conjugated secondary, and the staining is usually pretty
bright. But then again, my GFP expression is high.

Adam

On Thu, Oct 7, 2010 at 7:44 AM, Susan Travers <travers.3 <@t> osu.edu> wrote:

> Does anyone have experience with detecting EGFP with some of the current
> aby's made in chicken?  We have used aby's from both AVES labs and Millipore
> with negative results. In both cases we used the biotinylated secondary aby
> from AVES followed by a fluorescent streptavidin.  Absolutely no staining.
>
> Using the same tissue, we were able to use a different aby made in rabbit
> and it worked great. However, because of double-labeling needs we'd really
> like to get the chicken to work.
>
> Perhaps someone has experience or insights?
>
> Thanks!
> Susan Travers
> Division of Oral Biology
> College of Dentistry
> The Ohio State University
>
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