[Histonet] chicken EGFP aby
anonwums1 <@t> gmail.com
Thu Oct 7 08:23:16 CDT 2010
I've had good luck using Abcam's chicken polyclonal:
I use a directly conjugated secondary, and the staining is usually pretty
bright. But then again, my GFP expression is high.
On Thu, Oct 7, 2010 at 7:44 AM, Susan Travers <travers.3 <@t> osu.edu> wrote:
> Does anyone have experience with detecting EGFP with some of the current
> aby's made in chicken? We have used aby's from both AVES labs and Millipore
> with negative results. In both cases we used the biotinylated secondary aby
> from AVES followed by a fluorescent streptavidin. Absolutely no staining.
> Using the same tissue, we were able to use a different aby made in rabbit
> and it worked great. However, because of double-labeling needs we'd really
> like to get the chicken to work.
> Perhaps someone has experience or insights?
> Susan Travers
> Division of Oral Biology
> College of Dentistry
> The Ohio State University
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