[Histonet] PAS STAIN

[gayle callis]

I suspect bad Periodic acid.  We never buy periodic acid already in solution and if that has been sitting around even in a kit, it may have gone bad.  In a workshop taught by Charles Culling, an expert on PAS staining, he stressed making periodic acid fresh daily or each time the stain is done.  It is not expensive, goes into solution readily, then toss the periodic acid to never be reused with exception of that one day. Also, you can test your Schiffs reagent by putting a few drops of Schiffs into 10 mls NBF, watch it turn a very bright pink red instantly.   If it turns purplish, it is not good. It may be a bad kit, but you never said you were using a kit only "new bottles of ....."

Consequently we buy periodic acid in crystalline form, make up 1% Periodic acid, and buy the Schiffs Reagent from Fisher, never a kit.  Sigma also sells good Schiffs, but Fisher Scientific aka Thermo has larger quantity for less price.  We also store our Schiffs in the refrigerator rather than room temperature, a habit left over from days when we made this reagent up in house.  

Gayle M. Callis 
HTL/HT/MT(ASCP)  



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, October 06, 2010 9:06 AM
To: Diana McCaig; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS STAIN

Were the new bottles from the same lot? 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Diana
McCaig
Sent: Wednesday, October 06, 2010 11:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAS STAIN


Hi
We have been doing a PAS stain on the same control block and same
reagent supplier for a long time.  
Yesterday when we ran the slides, they failed to stain
 
We opened new bottles of Periodic Acid and Schiff's Reagent and recut
fresh controls
 
Still, we are unable to get any staining.
 
Suggestions to help would be appreciated
 
Diana
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