[Histonet] RE: embedding beads/tissue marking dyes

[Maria Katleba]

When you place dye on any tissue, make sure you pipette some bouins on to the tissue... Apparently the bouins "sets" the stain... afterwards dab the tissue with paper towel to sop up the excess dye/bouins.

Some dyes are better than others, but try the bouins first
Call me or email me directly, I can help you with details :)

Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth
Sent: Tuesday, October 05, 2010 2:14 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] embedding beads/tissue marking dyes

Hi
We may be encountering some issues with our tissue marking dyes 'clogging' up our processors.  I"ve heard of a system where a coloured 'bead' is embedded right beside the tissue, and subsequently cut onto the slide.  This does not mark the margins obviously, but is used as a method to track like specimens that are grossed, embedded and subsequently cut in a row. (red, orange, green, blue.....)

I explored the archives for "embedding beads", but only found reference to a glass bead that is placed in the wax inside the block, but not subsequently cut.

PS: we are still troubleshooting our dyes (dilutions, brand, etc.), but if anyone has a brand of dye that they like and have no issues with, I'd be thrilled to get the information as well!

Thanks in advance,
Jacquie

Jacqueline Farnsworth
Anatomic Pathology, Tech III
Diagnostic Scientific Centre
Calgary Laboratory Services

Phone: 403-770-3588
Pager: 403-212-8223  X07630

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