[Histonet] IHC unfixed human brain tissue

Connolly, Brett M brett_connolly <@t> merck.com
Mon Oct 4 14:50:20 CDT 2010


You need to do some fixation. Hopefully they are mounted on charged

We routinely post-fix fresh-frozen human brain sections in cold
Acetone/ETOH (3:1) for 10 min and then proceed with the IHC staining. 


Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Neil M.
Sent: Monday, October 04, 2010 3:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC unfixed human brain tissue

I have limited experience working with human brain tissue and would like
stain some human brain sections that I received. They were not fixed,
but were
flash frozen and sectioned on a cryostat, and mounted onto slides.

Could someone give me a brief run down of their standard methodological
procedure with unfixed human brain sections?

Much appreciated


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