[Histonet] IHC unfixed human brain tissue

[Neil M. Fournier]

I have limited experience working with human brain tissue and would like to
stain some human brain sections that I received. They were not fixed, but were
flash frozen and sectioned on a cryostat, and mounted onto slides.

Could someone give me a brief run down of their standard methodological staining
procedure with unfixed human brain sections?

Much appreciated

Neil