[Histonet] Vibratome sectioning of lung

Monfils, Paul PMonfils <@t> Lifespan.org
Tue Nov 30 14:00:39 CST 2010


Does anyone have experience sectioning agarose-embedded lung sections on
the Vibratome?  I have cut sections of various tissues on the Vibratome,
but am having a very difficult time with mouse lung.  The agarose does
not seem to adhere to the tissue, and the blade simply pulls the tissue
out of the agarose block.  Any suggestions will be most appreciated.



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