[Histonet] CD20 for IHC on mouse FFPE tissue

[Connolly, Brett M]

Thanks Guys,
I  plan to order one of the Rb monoclonals for CD20.

Best,
Brett

-----Original Message-----
From: Amos Brooks [mailto:amosbrooks <@t> gmail.com] 
Sent: Friday, November 26, 2010 4:58 PM
To: koellingr <@t> comcast.net
Cc: Connolly, Brett M; histonet <@t> lists.utsouthwestern.edu; Peter.Tree <@t> abdserotec.com
Subject: Re: [Histonet] CD20 for IHC on mouse FFPE tissue

Thanks Brett,
	Looks like I got a couple lines crossed there. I had been told that there 
wasn't any CD20 in mice. I have been using B220 for BCell detection. After you 
pointed out the error I looked it up at Biocompare and clearly there are some 
CD20 markers for mice. http://tinyurl.com/38wyh42
	I was also apparently wrong about BD being a Thermo company.  Thanks 
Peter. They do distribute it, but don't own it. My mistake ... again :-) With 
those goofs I'll shut up now and blame the turkey

Enjoy your weekend guys,
Amos


On Friday 26 November 2010 04:08:06 pm koellingr <@t> comcast.net wrote:
> Hello,
>
> Maybe I'm remembering wrong since I deleted the original message of Bretts
> looking for murine CD20 on mouse FFPE (hopefully rabbit origin?). If so
> anti-murine CD20 that can be made to work on mouse  FFPE tissues certainly
> exist. Have used them from BD (flow reagent), Santa Cruz and others.  In
> Biocompare website, can see them plus new rabbit monoclonal anti-murine
> CD20 for paraffin, reactive to murine tissues.  There are a few CD20+
> murine B-cells that have no B220, and vice-versa so I would'nt look for
> CD45R if I wanted to find CD20+ cells. Would look for CD20+ cells. Maybe
> I'm remembering original post incorrectly.
>
> Ray
>
>
>
> Ray Koelling
>
> PhenoPath Labs
>
> Seattle, WA
>
>  
> ----- Original Message -----
> From: "Amos Brooks" <amosbrooks <@t> gmail.com>
> To: "brett connolly" <brett_connolly <@t> merck.com>,
> histonet <@t> lists.utsouthwestern.edu Sent: Thursday, November 25, 2010 3:31:27
> AM
> Subject: [Histonet] CD20 for IHC on  mouse FFPE tissue
>
> Hi Brett,
>         CD20 is not going to work for mouse tissue. You need CD45R (clone
> B220). This will selectively label B cells just like CD20 in humans. I get
> this from BD Biosciences (Now Thermo ... again) Catalog # 347460. It is
> raised in Rats and works great as long as you have a good rat secondary.
> The one from Jackson is good.
>
> Happy Thanksgiving,
> Amos
>
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