[Histonet] Re: Histonet Digest, Vol 84, Issue 28
marilyngamble <@t> comcast.net
marilyngamble <@t> comcast.net
Thu Nov 25 22:04:01 CST 2010
----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 84, Issue 28
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Today's Topics:
1. Polyoma controls (Justin Peters)
2. Re: H pylori control on ventana immuno stainer (Mark Tarango)
3. RE: Rusting in Pathology Department (Laurie Colbert)
4. Re: Rusting in Pathology Department (Markus F. Meyenhofer)
5. GU pathology laboratory looking for histotechs/ cytotechs in
Columbus, Ohio (Histology Director)
6. HT Position (Hale, Meredith)
7. RE: ThinPrep vs. SurePath (Feher, Stephen)
8. C3D IF (Nails, Felton)
9. HistoGel for cell blocks (Bell, Lynne)
----------------------------------------------------------------------
Message: 1
Date: Mon, 22 Nov 2010 13:30:58 -0500
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] Polyoma controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24D22DE9E488AA43BF92A4389F2DDB1F15271354 <@t> mail1.BOSTWICK.COM>
Content-Type: text/plain; charset="us-ascii"
Does anyone know where I can get controls for polyomavirus (SV-40 large
T-antigen)?
Justin Peters HTL, QIHC (ASCP)CM
IHC Supervisor
Bostwick Laboratories(tm)
For Absolute Confidence(r)
4355 Innslake Drive
Glen Allen, Virginia 23060
Phone: (804) 967-9225 ext. 1831
Cell: (804) 822-6084
Email: jpeters <@t> bostwicklaboratories.com
<mailto:jpeters <@t> bostwicklaboratories.com>
------------------------------
Message: 2
Date: Mon, 22 Nov 2010 10:41:43 -0800
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] H pylori control on ventana immuno stainer
To: Gloria Cole <gloria.cole <@t> usa.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTi=wQC7ykt2X0eqtRsg6P4X00CaOE9JZhB=z=v1e <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Gloria,
Are you still looking for some good HP controls? I could send you a block
of one of our controls if you're intersted. I have blocks from
several different specimens. Just let me know your address and fedex
number.
Thanks,
Mark
On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole <gloria.cole <@t> usa.net> wrote:
> Hi,
>
> I am setting up a new lab and I am using the Ventana immuno stainer, does
> anyone know of any good HP controls I can purchase for now that will work
> well with the Ventana?
>
> Thanks,
>
> Gloria
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 3
Date: Mon, 22 Nov 2010 13:08:59 -0800
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: RE: [Histonet] Rusting in Pathology Department
To: <Beth.Fye <@t> HCAhealthcare.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<57BE698966D5C54EAE8612E8941D768309FF69EC <@t> EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="us-ascii"
How about decal solution (acid)? Do you have open containers at the
microtomes that the histotechs use throughout the day?
Straight acids will cause metal to rust. We have an acid storage
cabinet that is wood, but the hinges and handles are totally rusted out.
Laurie
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Beth.Fye <@t> HCAhealthcare.com
Sent: Thursday, November 18, 2010 5:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rusting in Pathology Department
Here is a question I don't believe I ever seen discussed here before.
Does anyone else have significant rusting issues in their Histology
Departments? Cabinet hinges, metal outlet plates, printers, etc. are
rusting in our department, especially around the microtome areas.
Please let me know if you are having any of these issues, or can think
of any reason for this. We had a printer in the lab that the whole
interior rusted out. This is not minor rusting that I could contribute
to water bath humidity.
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
fax: (804)323-8638
<mailto:Beth.Fye <@t> hcahealthcare.com>
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------------------------------
Message: 4
Date: Mon, 22 Nov 2010 16:23:01 -0500
From: "Markus F. Meyenhofer" <micro <@t> superlink.net>
Subject: Re: [Histonet] Rusting in Pathology Department
To: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>,
<Beth.Fye <@t> HCAhealthcare.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <95E49A4ECF834A5DA7DD40B2EBD0DA43 <@t> DJ4VDH31>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
It's the fumes of Hydrochloric Acid from solutions.
----- Original Message -----
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
To: <Beth.Fye <@t> HCAhealthcare.com>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, November 22, 2010 4:08 PM
Subject: RE: [Histonet] Rusting in Pathology Department
How about decal solution (acid)? Do you have open containers at the
microtomes that the histotechs use throughout the day?
Straight acids will cause metal to rust. We have an acid storage
cabinet that is wood, but the hinges and handles are totally rusted out.
Laurie
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Beth.Fye <@t> HCAhealthcare.com
Sent: Thursday, November 18, 2010 5:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rusting in Pathology Department
Here is a question I don't believe I ever seen discussed here before.
Does anyone else have significant rusting issues in their Histology
Departments? Cabinet hinges, metal outlet plates, printers, etc. are
rusting in our department, especially around the microtome areas.
Please let me know if you are having any of these issues, or can think
of any reason for this. We had a printer in the lab that the whole
interior rusted out. This is not minor rusting that I could contribute
to water bath humidity.
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
fax: (804)323-8638
<mailto:Beth.Fye <@t> hcahealthcare.com>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 5
Date: Tue, 23 Nov 2010 08:34:29 -0500
From: Histology Director <histologydirector <@t> gmail.com>
Subject: [Histonet] GU pathology laboratory looking for histotechs/
cytotechs in Columbus, Ohio
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AANLkTinLUTJ7B655yg7ErhPiUNeuQfze-qpyQ5pwB1uF <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to careers <@t> aksm.com.
------------------------------
Message: 6
Date: Tue, 23 Nov 2010 08:41:01 -0600
From: "Hale, Meredith" <mhale <@t> carisls.com>
Subject: [Histonet] HT Position
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6F33D8418806044682A391273399860F0610502E <@t> s-irv-ex301.PathologyPartners.intranet>
Content-Type: text/plain; charset="us-ascii"
Great opportunity for a Histotechnician in a brand new laboratory!
Bellmeade Dermatology in Nashville, TN is looking for a certified HT or
HTL to run their newly constructed laboratory. Bellmeade Dermatology has
been in the dermatology business for 18 years with 3 physicians and 2
Nurse Practitioners' . Candidate must be ASCP certified and CLIA
certified to perform gross dissection, prior supervisory experience
preferred. The candidate will be responsible for the following: Creation
and maintenance of policies and procedures to CLIA standards, leading
lab through CLIA inspection, maintenance and quality control for
equipment, and routine histology duties. This is a part time position
that offers a competitive salary and the flexible hours allows you to
put your own personal stamp on the laboratory . Interested applicants
should contact Meredith Hale phone 214-596-2219 or through email
mhale <@t> carisls.com
Meredith Hale HT (ASCP) CM
Operations Liaison Director and Education Coordinator
Caris Life Sciences
6655 North MacArthur Blvd, Irving Texas 75039
direct: 214-596-2219
cell: 469-648-8253
fax: 972-929-9966
mhale <@t> carisls.com <mailto:mhale <@t> carisls.com>
------------------------------
Message: 7
Date: Tue, 23 Nov 2010 09:50:00 -0500
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] ThinPrep vs. SurePath
To: "Jennifer MacDonald" <JMacDonald <@t> mtsac.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0908FC0A43B87A4FB60EDCCA06AABC243868BA <@t> exchange.cmc-nh.org>
Content-Type: text/plain; charset="us-ascii"
Hi Jennifer,
I have used both systems, as a cytotech, processing tech, and as a
supervisor. As a cytotech, there is less area of the slide to have to
review so from that viewpoint it seems more efficient. The drawback for
me was the size of the cells. For Gyn specimens, very small high grade
cells can be difficult to detect without some sort of computer assisted
system. The Focal Point system was supposed to provide this assistance
but I found it somewhat unreliable. This was further complicated by the
Focal Point being about 50% accurate in detecting endocervical cells.
Labs that allowed specimens that automatically signed out cases the
Focal Point deemed at the lowest risk for abnormal cells frequently had
clinicians repeating paps due to there being no endocervical material
detected. I found that non-gyn specimens processed using SurePath were
very difficult for inexperienced techs and many pathologists simply did
not trust that they were getting the appropriate sampling of cells.
Very experienced cytotechs and some pathologists preferred SurePath for
non-gyns but they are in the minority and I found them difficult and
time consuming to review. As a processing tech, SurePath was very time
consuming and if a lab has any kind of volume at all, had to have at
least 2 technicians dedicated to nothing but processing them.
ThinPrep has a larger are to look at and those techs who are used to
SurePath do not like to review them because the cells are quite a bit
larger, and if doing manual screening, takes a bit longer to review.
Use of the ThinPrep Imager has cut screening time down considerably.
Time and statistical analysis has shown that the Imager is a very
reliable instrument for indicating those areas of the slide where
abnormal cells may be found. Criticism has been that the Imager
sometimes misses cells with HPV and viral effect. Even so, the primary
focus of many labs is to properly detect and report high grade lesions
and for this the Imager is reliable. For me, non-gyn specimens were
easier to review and displayed a good distribution of cells. A good
many pathologists agree and are confident that the cell sampling
adequate. As a processing tech, ThinPrep processing takes less time and
less personnel than SurePath. If the T-5000 processor is ever released
by the FDA for use in the US for gyn specimens, this time will be
further reduced.
In the end, the smaller cell distribution size offered by SurePath,
while seemingly more efficient, requires more time to review for many
techs due to the small size of the cells. ThinPrep currently offers a
better solution to a majority of labs in terms of accuracy, time to
prepare, and Imaging.
Hope this was helpful.
Steve
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Wednesday, November 10, 2010 6:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ThinPrep vs. SurePath
Does anyone have an opinion as to why there seems to be many more
ThinPreps out there than Sure Path? The SurePath seems more efficient,
but I might be missing something.
Thank you,
Jennifer MacDonald
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 23 Nov 2010 10:28:23 -0600
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: [Histonet] C3D IF
To: "'Laurie Colbert'" <laurie.colbert <@t> huntingtonhospital.com>,
"godsgalnow <@t> aol.com" <godsgalnow <@t> aol.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1FE5960057C084CA389CE97779062908E717C0C <@t> TCDMSG01.ad.TexasChildrensHospital.org>
Content-Type: text/plain; charset=us-ascii
Is anyone perform immunofluorescence for C3D?
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Message: 9
Date: Tue, 23 Nov 2010 12:09:09 -0500
From: "Bell, Lynne" <Lynne.Bell <@t> cvmc.org>
Subject: [Histonet] HistoGel for cell blocks
To: "Histonet (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F9E5562685AF9B498D9D8C1EC996D2440F5DA6EAB4 <@t> cvmc-email.CVMC.ORG>
Content-Type: text/plain; charset="us-ascii"
One of my pathologists is interested in the pros and cons of HistoGel for cell blocks. I have read the archives and gathered some information for him. He specifically would like to talk to someone that has been using it for some time.
Along the same line, what method do you find to be the best for cell blocks?
Any help would be appreciated. Thanks!
Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
P. O. Box 547
Barre, VT 05641
802-371-4923
------------------------------
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