[Histonet] 70% alcohol

[Molinari, Betsy]

That is how I handle ours as well. Start in 95% and proceed with the normal processing protocol.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524	
832-355-6812 (fax)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 23, 2010 3:54 PM
To: histonet <@t> lists.utsouthwestern.edu; sgoebel <@t> mirnarx.com
Subject: Re: [Histonet] 70% alcohol

It is always better to be safe than sorry. You should start in EtOL95% → 100% and clear as usual before infiltration.
René J.

--- On Tue, 11/23/10, sgoebel <@t> mirnarx.com <sgoebel <@t> mirnarx.com> wrote:


From: sgoebel <@t> mirnarx.com <sgoebel <@t> mirnarx.com>
Subject: [Histonet] 70% alcohol
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, November 23, 2010, 4:48 PM


Hello all!!

I moved jobs, but am still here in 80 degree Austin weather...

Question.  If tissue has been stored in 70% alcohol for up to a year,
does there need to be anything done to it before processing?  I am
assuming that some rehydration might be necessary?  If not can you just
throw it on  the processor on a normal cycle?  Thanks for you help!!



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912



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