[Histonet] Nails

[Patsy Ruegg]

I have used elmer's glue coated plain slides for years for tissues difficult
to keep on the slides, such as cartilage, bone, nails, etc.  I make a 5%
solution of the glue in dih20 (about 200 mls) and then load racks (24 slots)
with slides and dip them in the glue solution about 10 times then take the
slides out of the racks and line them up around something to airdry, usually
overnight, you can then store the dried slides next to each other back in
slide boxes for future use.  After they are dry I do not think they have a
shelf life as I have used slides that are more than a year old and they
still work, just the quick dip in the waterbath to pick up the section seems
to activate the glue enough to make the sections stick well.  One cautionary
thing to keep in mind is that the glue itself can be bio reactive with
certain IHC protocols as it may have some ridicule Ig in the glue if it is
made from animals.

Regards,

Patsy   

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Friday, November 19, 2010 9:51 AM
To: 'Shirley A. Powell'; godsgalnow <@t> aol.com;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails

An old old trick is taking a plain slide and coating it with dilute Elmer's
glue and letting it dry. Be quick picking it up from the water bath and let
dry well.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shirley A.
Powell
Sent: Friday, November 19, 2010 11:40 AM
To: godsgalnow <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails

Switch to Stay On and plain slides, I had problems with charged slides and
now have good results with the additive and regular slides.  Cheaper in the
long run too.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
godsgalnow <@t> aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides
when we do the PASF stain.  We are using charged slides already and we have
tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



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