[Histonet] Nails

[Maria Katleba]

How To Cut Nails
1. section nail and place on superfrost plus (or adhesive slide)
2. shake excess water off slide
3. place 1-3 drop formaldehyde (10% buffered ok) at the bottom of a plastic coplin jar
4. place cut slides in jar
5. tighten cap
6.Place on hot over 60 degrees...15 -20 minutes
7. remove (do NOT rinse) and cool
8. stain or deparifinize

The aldehyde in the formalin moves through the tissue and with the heat, it vaporizes... it pushes the tissue against the glass and it becomes 'set' almost permanently!


Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707) 294-9229 cell- anytime
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of godsgalnow <@t> aol.com
Sent: Friday, November 19, 2010 8:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when we do the PASF stain.  We are using charged slides already and we have tried increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



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