[Histonet] Tissue loss and FISH

BSullivan <@t> shorememorial.org BSullivan <@t> shorememorial.org
Wed Nov 17 06:41:34 CST 2010

I have to agree with Sheila's statement. When we experience this during
fish or immuno staining my first thing to check is temperature and the next
thing is the slides. The slides almost always is the culprit.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital

Speak only well of people and you need never whisper

             Sheila Haas                                               
             <micropathlabs <@t> ya                                         
             hoo.com>                                                   To
             Sent by:                  "Kuhnla, Melissa"               
             histonet-bounces@         <Melissa.Kuhnla <@t> chsli.org>, 
             lists.utsouthwest         histonet <@t> lists.utsouthwestern.edu
             ern.edu                                                    cc
             11/17/2010 07:34          Re: [Histonet] Tissue loss and FISH

We recently had problems with our Superfrost Plus slides that we purchase
Cardinal. We had sections washing off and uneven staining. We switched to
VistaVision Histobond Slides and have had no further issues. 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc. 

From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, November 17, 2010 7:28:53 AM
Subject: [Histonet] Tissue loss and FISH

We are a lab that has been running Pathvysion Her2 FISH for years
without any major problems.  Recently we have been experiencing more and
more tissue section loss. I have noticed that some of the loss is
starting during pretreatment. My temperatures for all steps are what
they should be.  During each temp. dependent step, we have two
thermometers giving similar readings. Nothing has changes regarding our
slide baking.  I have a call into the vendor of the plus slides to
verify the integrity of the lot.  I have recently noticed the labeling
on the pathvysion bottles is slightly different; I also have a call into
them as well.

Does anyone have any thoughts?  Does anyone know of processing variables
that may have lead to this????
Thank you,

The information in this e-mail, and any attachments therein, is
confidential and
for use by the intended addressee only. If this message is received by you
error please do not disseminate or read further. Please reply to the sender
you have received the message in error, then delete the message. Although
Catholic Health Services of Long Island attempts to sweep e-mail  and
attachments for viruses, it does not guarantee that either are virus-free
accepts no liability for any damage sustained as a result of viruses. Thank

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Copyright 2007 Shore Memorial Hospital

More information about the Histonet mailing list