[Histonet] Tissue loss and FISH

[BSullivan <@t> shorememorial.org]

I have to agree with Sheila's statement. When we experience this during
fish or immuno staining my first thing to check is temperature and the next
thing is the slides. The slides almost always is the culprit.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


Speak only well of people and you need never whisper


                                                                       
             Sheila Haas                                               
             <micropathlabs <@t> ya                                         
             hoo.com>                                                   To
             Sent by:                  "Kuhnla, Melissa"               
             histonet-bounces@         <Melissa.Kuhnla <@t> chsli.org>, 
             lists.utsouthwest         histonet <@t> lists.utsouthwestern.edu
             ern.edu                                                    cc
                                                                       
                                                                   Subject
             11/17/2010 07:34          Re: [Histonet] Tissue loss and FISH
             AM                                                        
                                                                       
                                                                       
                                                                       
                                                                       
                                                                       




We recently had problems with our Superfrost Plus slides that we purchase
from
Cardinal. We had sections washing off and uneven staining. We switched to
VWR
VistaVision Histobond Slides and have had no further issues. 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc. 




________________________________
From: "Kuhnla, Melissa" <Melissa.Kuhnla <@t> chsli.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, November 17, 2010 7:28:53 AM
Subject: [Histonet] Tissue loss and FISH

Hello,
We are a lab that has been running Pathvysion Her2 FISH for years
without any major problems.  Recently we have been experiencing more and
more tissue section loss. I have noticed that some of the loss is
starting during pretreatment. My temperatures for all steps are what
they should be.  During each temp. dependent step, we have two
thermometers giving similar readings. Nothing has changes regarding our
slide baking.  I have a call into the vendor of the plus slides to
verify the integrity of the lot.  I have recently noticed the labeling
on the pathvysion bottles is slightly different; I also have a call into
them as well.

Does anyone have any thoughts?  Does anyone know of processing variables
that may have lead to this????
Thank you,
Melissa


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