[Histonet] Tap Water or filtration system

Feher, Stephen sfeher <@t> CMC-NH.ORG
Mon Nov 15 16:45:24 CST 2010

We use tap water in our Leica multistainer for H&E's and a few other
stains.  The protocols can be optimized to accommodate what ever you are
using. I can't speak to the PAS stain except to say that even if you
need deionized water, you can buy it in 5 gallon cubes for limited use
and still save money.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa
Sent: Monday, November 15, 2010 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tap Water or filtration system

We are in the process of getting our new Lecia Autostainer delivered to
The question came up if we will still need to continue using our water
purification system for the stainer. Mostly the docs would like to save
money but I don't want them to be unhappy with the result.


I would like to hear all the pros and cons of discontinuing/continuing
the use of our system and going w/ tap water from the sink. What is the
PH level supposed to test at? What difference will I see in the slides?
By the way, I only stain H&E  at the current time but there is a
possibility of starting PAS or other special stains.


Thank you in advance!!!







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