[Histonet] rapid h and e

Brandi Higgins brandihiggins <@t> gmail.com
Fri Nov 12 22:25:37 CST 2010

We used to do the similar to what Bill Tench described below.  However,
destaining and restaining with Pap caused loss of some of the cells and the
pathologists prefer the pap for these smears so we devised a Stat Pap
stain:  from alcohol, 50% alcohol, water, Gil's Hematoxylin for 1 min,
water, bluing, water, 50% alcohol, alcohol OG6 10 dips alcohol EA50 10 dips
alcohol, clearing agent, coverslip.  The pathologists have been really happy
with this, we've been using it for about 7 months no with no complaints
other than once they said stain was too dark, but smear was too thick in
some areas.  Hope this helps.

Brandi Higgins

On Fri, Nov 12, 2010 at 7:00 PM, Tench, Bill <Bill.Tench <@t> pph.org> wrote:

> We use the same materials and timing we use for a frozen section, which
> as a cytopathologist works just fine for me.  Approx 1 min Hematox,
> several dips wash, dips in blueing UNTIL BLUE (people tend to shorten
> this critical step), no more than 5 dips in eos, 100 ETOH X 2, xylene
> dipped until runs smooth, coverslip.  Takes about 1 min 45 sec.  If
> there was a lot of blood, one can start with quick fix and dips in acid
> alcohol, or the histo people can destain and restain the case at a later
> date.
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench <@t> pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
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