[Histonet] rapid h and e

Tench, Bill Bill.Tench <@t> pph.org
Fri Nov 12 18:00:14 CST 2010

We use the same materials and timing we use for a frozen section, which
as a cytopathologist works just fine for me.  Approx 1 min Hematox,
several dips wash, dips in blueing UNTIL BLUE (people tend to shorten
this critical step), no more than 5 dips in eos, 100 ETOH X 2, xylene
dipped until runs smooth, coverslip.  Takes about 1 min 45 sec.  If
there was a lot of blood, one can start with quick fix and dips in acid
alcohol, or the histo people can destain and restain the case at a later
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

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