[Histonet] CK7/EMA control tissue

[Lee & Peggy Wenk]

Some lung carcinoma could be positive for both.

Since IHC is usually done on tissue with cancer, which often has low levels 
of antigens, I personally would rather use another cancer as a control, 
rather than a "normal/benign" tissue which would have high levels of the 
antigen. More likely to miss the low levels in patient tissue when using a 
too positive control.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

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From: "David Hill" <dhill32 <@t> comcast.net>
Sent: Thursday, November 11, 2010 9:51 PM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] CK7/EMA control tissue

> I'm looking to find some control tissue that will stain positive for ck7 
> and ema. We are currently using breast carcinoma tissue but most of the 
> tumors we are seeing upon grossing are being submitted entirely for 
> pathologist review. I have been searching the literature for any normal 
> tissue that stains for ck7 and ema with no luck. We use a multi control 
> block for our immuno's so I would like to find something common and 
> normal/benign to use. Any help or suggestions would be appreciated.
>
> Thanks,
> David Hill
> Trumbull Labs
> Germantown, TN
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