[Histonet] unstained paraffin tissue slides storage--why cold?

[Jan Shivers]

We keep all of our control blocks and slides at 4C in plastic boxes, and 
date all unstained slides with date of sectioning.

Jan Shivers
UMN Vet Diag Lab
St. Paul, MN


----- Original Message ----- 
From: "Goins, Tresa" <TGoins <@t> mt.gov>
To: <sgoebel <@t> xbiotech.com>; "Emily Sours" <talulahgosh <@t> gmail.com>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, November 04, 2010 11:41 AM
Subject: RE: [Histonet] unstained paraffin tissue slides storage--why cold?


> For storage of multiple IHC control blocks - we store at room temperature 
> but cut from only one in the series at a time.  After the tissue is 
> verified as a good positive control, the cut surface of the block is 
> "painted over" with melted paraffin from the embedding station to prevent 
> exposure to the air.
>
>
> Tresa Goins
> Veterinary Diagnostic Lab
> Department of Livestock
> Bozeman, Montana
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> sgoebel <@t> xbiotech.com
> Sent: Thursday, November 04, 2010 9:21 AM
> To: Emily Sours
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] unstained paraffin tissue slides storage--why 
> cold?
>
> -70 or -80 seems a little extreme to me, that's why I always just leave
> them in a normal freezer (-20).  I think the main point of doing this
> from what I understand is so that the antigens stay "viable".  I know
> over time they can degrade and so your stain won't work with some
> antibodies.  The weirdest part to me has always been that you don't have
> to store the blocks this way.  So I think that was your question, if the
> blocks aren't stored in a freezer why store the slides?  Won't the
> antigens in the blocks start to degrade as well?  This is a question I
> would like to know the answer to as well...
>
> Sarah Goebel, B.A., HT (ASCP)
> Histotechnician
>
>
> XBiotech USA Inc.
>
> 8201 East Riverside Dr. Bldg 4 Suite 100
>
> Austin, Texas  78744
>
> (512)386-2907
>
>
>
>
> -------- Original Message --------
> Subject: Re: [Histonet] unstained paraffin tissue slides storage--why
> cold?
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Date: Thu, November 04, 2010 7:07 am
> To: histonet <@t> lists.utsouthwestern.edu
>
> Can I ask what the point of storing paraffin sections in freezing cold
> storage?
> They are wax sections, which never see any type of cold, so I don't
> understand the point of this. I do understand putting them at 4 degrees
> to
> prevent mold, but -80 seems excessive.
> We have kept our slides at room temperature for years and years, but
> these
> slides do not have an albumin coat (which I can see getting moldy), just
> a
> chemical coating.
> Fixing for paraffin and paraffin infiltration seems to keep antigens
> safe
> without refrigeration because it's so intense, but that's just
> conjecture on
> my part.
>
> Emily
> --
> Outside of a dog, a book is man's best friend. Inside of a dog it's too
> dark
> to read.
> --Groucho Marx
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