[Histonet] IHC Staining Problem

[Amos Brooks]

Hi,
     I think you mentioned using citrate retrieval. Have you tried high pH
retrieval with EDTA or an equivalent? I think CD79 works better with high pH
retrieval.

Amos


Message: 1
Date: Sun, 31 Oct 2010 19:29:59 +0000
From: Jennifer Hill <jhill <@t> vet.k-state.edu>
Subject: RE: [Histonet] IHC Staining Problem
To: histonet <histonet <@t> lists.utsouthwestern.edu>
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       <8AA2173DC209CA438077A832FF98BD7F02806542 <@t> VETMXHT.ads.vet.k-state.edu
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Thanks to everyone who has responded so far.  To answer the questions asked,
our controls are either being cut the same day or the day or two before, so
they are not setting for extended periods.  We have also used at least three
or four different controls, all with the same results. (The controls stain
fine for other tests: CD20, CD3, etc.)The pathologists are saying that the
patient slides are not staining as well.  So it doesn't seem to be something
so simple as a control issue.  As to the dehydration/deparafinization, that
takes place on an automated stainer, and no other stains are having issues
so I don't believe that is the issue either.


Jennifer Hill
Kansas State University
Veterinary Diagnostic Lab