[Histonet] Nissl Stain P0 Mouse Head

[sgoebel <@t> xbiotech.com]

   Did you decal the skull?

   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech USA Inc.

   8201 Eas
   
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Nissl Stain P0 Mouse Head
   From: Kevin Gillinder <[1]   Date: Fri, May 21, 2010 5:00 am
   To: "Histonet <@t> lists.utsouthwestern.edu"
   <[2]Histonet <@t> lists.uts   Hi,
   I  am  having  some  trouble  with  Nissl  staining newborn (P0) mouse
   heads/hindb   The  nissl  stain  appears  very  faint  in the centre of my sections.
   (Paraffin    Is this likely to be a penetration issue?
   I fixed in 4% PFA O/N before processing - Would that affect it?
   I  left the skull intact, but removed the skin - should I have removed
   some    Any help appreciated!!!!!!
   Regards,
   --
   Kevin
   _______________________________________________
   Histonet mailing list
   [3]Histonet <@t> lists.utsouth   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. 3D"mailto:k.r.gillinder <@t> newcastle.ac.uk"
   2. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
   3. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
   4. 3D"http://lists.utsouthwestern.edu/mailman/listin