[Histonet] possible AFB contaminant

[Rena Fail]

Are you using tubing on your dispensers for your DI water? They can be a source of contaminant. You can also have cross contaminantion from your control slide during dehydration and hydration as well as during staining.Do not use the same set up for contrpl and pt slide. Lay slides flat for hand staining
Rena Fail



----- Original Message ----
From: "Debra.Ortiz <@t> uchospitals.edu" <Debra.Ortiz <@t> uchospitals.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thu, May 20, 2010 3:41:09 PM
Subject: [Histonet] possible AFB contaminant

Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are "false positives" on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi

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University of Chicago Medical Center 
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