[Histonet] possible AFB contaminant

[Mike Pence]

We decontame once a month.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Thursday, May 20, 2010 3:23 PM
To: Debra.Ortiz <@t> uchospitals.edu; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] possible AFB contaminant


How often are you decontaminating your NeXes machines (running 
decontaminating reagents through the tubing)?

Jan Shivers
UMN VetDiagLab

----- Original Message ----- 
From: <Debra.Ortiz <@t> uchospitals.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, May 20, 2010 2:41 PM
Subject: [Histonet] possible AFB contaminant


Please help, we have had an increase in demands for repeats on our AFB 
stain. Our pathologists have been noticing what they believe are "false 
positives" on our slides. We perform our AFBs on the Ventana Nexus and
some 
are done by hand. Because of this problem, we have been doing side by
side 
comparisons using the Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits,
tested 
our DI water sytem and have different techs cutting using different 
waterbaths.
Any advice or comments would be greatly appreciated.

Thanks Debi

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Thank you
University of Chicago Medical Center
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