[Histonet] AANLkTik8dnsQt16RPMSS8_x5fgqQE_18-ctUeNp71V0L@mail.gmail.com

[louise renton]

My wrong....I meant epidermis. Blame old age




On Thu, May 20, 2010 at 12:08 AM, Andrew Burgeson <napoli <@t> siscom.net> wrote:

> "One thing i forgot to mention wasthat when you embed, try
> to orientate the
> tissue so that the long axis (if there is one) lies in the
> same direction as
> the cutting stroke. when embedding, orientate the tissue at
> a slight
> diagonal, so that the knife dous not continously pass
> through the tissue on
> the cutting stroke -
>
> (this works well for skins also, except make sure the
> dermis is away from the knife)
>
>
> I do not agree with the above statement about the "dermis
> being embedded so as to be facing away from the blade." The
> last tissue to hit the knife edge should be EPIDERMIS.
> Dermis and SubQ fat should be the first tissues to hit the
> blade. Perhaps this is what you meant by "dermis?"
> Otherwise, I would agree with that methodology of
> orientation and angle.
>
> MethylMethacrylate bone embedding works very well from what
> I understand. See link:
>
> http://www.jhc.org/cgi/content/full/45/2/307
>
>
>
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
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