[Histonet] Fwd: TDE decalcifier, decalcification commentary

[louise renton]

 Thanks Gayle for your input.

Trouble is, i like the TDE system, and despite not knowing what's in the
solution the IHC results are great. I wasn't sure if endpoint testing could
be used with other acids besides HCl, but you have answered my doubts on
that score. I suspect that, from the smell, it is HCl, but I will have to
dig out old chemistry books to remember how to determine an unknown acid!

anyway, thanks again for your invaluable advice.

best regards






On Tue, May 18, 2010 at 8:52 PM, gayle callis <gayle.callis <@t> bresnan.net>wrote:

>  Hi Teri and Louise,
>
>
>
> There is NO way to tell if a decalcifying solution is used up but you can
> be sure it IS used up.  So the best thing to do is endpoint testing to know
> when the bone is free of calcium.  All decalcifiers become exhausted, so one
> has to replenish the solution with fresh throughout the decal procedure,
> faithfully.  This really doesn't take that much time in the long run, and
> guarantees properly decalcified bone that will not be overexposed to acid -
> a huge no no for decent staining.
>
>
>
> The weight loss/weight gain method is great if you don't have an xray
> machine.   If the solution is an acid e.g. nitric, HCl, or formic, then one
> should do the chemical test or you can play with the weight loss/weight gain
> method.
>
>
>
> Attached are both the chemical and weight loss/weight gain methods, the
> latter was developed for testing nitric acid.  However, this is the easiest
> method to test EDTA other than an xray machine, with the latter being the
> most sensitive i.e. accurate.
>
>
>
> *If the company cannot provide the answer for what is in TDE, then I would
> not use it*. MSDS sometimes tell you, but if not, that company would be
> sayonara from my lab.   I have to know WHAT chemical is doing the work, very
> important to do IHC or routine only staining.   I would rather make it up
> decalcifying solutions myself than buy an product that is unknown, and
> stubbornly proprietary!
>
>
>
> If you do a chemical endpoint test, do NOT stir the decal solution during
> decalcification.  Suspend bones, then collect the aliquot from bottom of the
> container where Ca++ resides.  Remove your samples, and rinse with tap water
> while you do the test.  Return samples to FRESH decalcifying solution and
> proceed with decalcification/testing, etc, etc.
>
>
>
> May not be the answers you wanted but certainly how I have always done bone
> decalcification without problems.
>
>
>
> Take care, Ladies
>
>
>
> Gayle Callis
>
>
>
>
>
>
>
>
>
> *From:* Johnson, Teri [mailto:TJJ <@t> stowers.org]
> *Sent:* Tuesday, May 18, 2010 9:58 AM
> *To:* 'gayle callis'
> *Subject:* Histonet post
>
>
>
> Hey Gayle, thought I'd point this one out to you. Looks like it's right up
> your alley.
>
>
>
> Message: 1
>
> Date: Mon, 17 May 2010 19:30:30 +0200
>
> From: louise renton <louise.renton <@t> gmail.com>
>
> Subject: [Histonet] TDE decalcifier
>
> To: histonet <@t> lists.utsouthwestern.edu
>
> Message-ID:
>
>         <AANLkTinRMd_lrg8oTwuaSHUNfc-unuv5x8fJViUDY7F0 <@t> mail.gmail.com>
>
> Content-Type: text/plain; charset=ISO-8859-1
>
>
>
> hi all,
>
> although I have been using the TDE decalcification system for awhile, I was
>
> hoping to get some answers from the community. are there any thoughts as to
>
> what the solution is?. And how do you tell if  the solution is "used up" or
>
> not. I wonder if anybody out there has some idea as to whether the solution
>
> is "saturated" with calcium salt or not. as always, I look forward to some
>
> answers.
>
> Best regards
>
> --
>
> Louise Renton
>
> Bone Research Unit
>
> University of the Witwatersrand
>
> Johannesburg
>
> South Africa
>
> +27 11 717 2298 (tel & fax)
>
> 073 5574456 (emergencies only)
>
> "There are nights when the wolves are silent and only the moon howls".
>
> George Carlin
>
> No trees were killed in the sending of this message.
>
> However, many electrons were terribly inconvenienced.
>
>
>
>
>
> I hope things are thawing a bit where you are. I wish it would quit raining
> and warm up here. Supposed to be 80 degrees this weekend. I can't wait!
>
>
>
> All the best,
>
> Teri
>
>
>
>
>
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-- 
 Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.